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KMID : 0545119950050060370
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 6 p.370 ~ p.372
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Cloning of Autonomously Replicating Sequence from Phaffia rhodzyma
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Chun Soon-Bai
Chun Seung-Hee
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Abstract
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A Phaffia rhodozyma chromosomal fragment (approximately 3.8 kb) capable of functioning as an origin for the replication of a kanamycin resistance (km´) plasmid in S. cerevisiae was isolated by the use of origin search plasmid, pHN134. In S. cerevisiae, transformation frequencies using the plasmid pHN134 containing an autonomously replicating sequence of P. rhodozyma was 450-580 CFU/㎍ DNA. The stability of the recombinant plasmid were 16-19%.
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